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School of Natural Sciences

University Of California Merced

Services

PLEASE NOTE - RECHARGE RATES NOW IN EFFECT (Memo regarding rates here)

Cost Per Sample:

UC Merced Campus -  $5.00
Other UC Campus -  $6.00
Outside Industry -  $7.50

SERVICES

  • DNA Sequencing
  • Colony Picking, Replicating, and Rearraying – (coming soon)
  • MicroArray Printing - (coming soon)

ABI 3130xl Genetic Analyzer
The genomics core facility uses an ABI Prism 3130xl Genetic Analyzer for all DNA sequencing. The ABI 3130xl is a medium throughput sequencer that has 16 capillaries with a capacity of running 16 samples simultaneously. The optimized 3130 POP 7 (Performance Optimized Polymer) modules result in more consistent, higher quality data across a wider range of sequencing and fragment analysis applications with faster turn-around times.

Genetix QBOT
The QBOT is a high-throughput robot system. Designed around a highly accurate (1 microM) triple axis, linear drive system, the QBOT combines speed, precision, versatility and durability to offer an invaluable tool for high-throughput. The system is fully automated and enclosed to prevent any type of contamination and ensure sample quality and reproducibility.

Functions:

  • Colony Picking: Can automatically pick in excess of 3,500 colonies
  • Gridding: Grids a maximum of 15 filters at a time, with each filter being divided into a maximum of six fields
  • Replicating: Compressing libraries held in 96 well plates into 384 well plates and vice versa
  • Rearraying: Rearray interesting wells from a library into new wellplates, eliminating manual fatigue and human error

DeRisi MicroArray Printer
The DeRisi MicroArray printer is a special purpose instrument designed to deposit high-density, gridded arrays of cDNA, genomic DNA or similar biological material on microscope slides. The principal components are a computer controlled high precision, high speed, three-axis robot and a unique pen tip assembly. It is capable of depositing thousands of extremely small droplets of genetic material on a small surface area, one droplet at a time, with each droplet containing a different gene. Such a capability permits gene expression experiments using tiny samples of genetic material while obtaining simultaneous data on thousands of genes.